Separation of Phosphatidylinositol 4-Phosphate from a Mixture with Phosphatidylserine and Phosphatidylinositol by Sep-Pak C18 Cartridge.
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چکیده
منابع مشابه
Phosphatidylinositol 4-phosphate and phosphatidylinositol 3-phosphate regulate phagolysosome biogenesis.
Professional phagocytic cells ingest microbial intruders by engulfing them into phagosomes, which subsequently mature into microbicidal phagolysosomes. Phagosome maturation requires sequential fusion of the phagosome with early endosomes, late endosomes, and lysosomes. Although various phosphoinositides (PIPs) have been detected on phagosomes, it remained unclear which PIPs actually govern phag...
متن کاملDistinct Golgi populations of phosphatidylinositol 4-phosphate regulated by phosphatidylinositol 4-kinases.
Phosphatidylinositol 4-phosphate (PI4P) regulates biosynthetic membrane traffic at multiple steps and differentially affects the surface delivery of apically and basolaterally destined proteins in polarized cells. Two phosphatidylinositol 4-kinases (PI4Ks) have been localized to the Golgi complex in mammalian cells, type III PI4Kbeta (PI4KIIIbeta) and type II PI4Kalpha (PI4KIIalpha). Here we re...
متن کاملEffects of phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol 4-phosphate on a Na+-gated nonselective cation channel.
Olfactory receptor neurons in the lobster express a nonselective cation channel that is activated by intracellular Na+ and carries a substantial part of the depolarizing receptor current. Here, we show that phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2] and phosphatidylinositol 4-phosphate [PI(4)P] applied to the intracellular face of cell-free patches activate the channel in the absence of ...
متن کاملAnalysis of the metabolic turnover of the individual phosphate groups of phosphatidylinositol 4 - phosphate and phosphatidylinositol 4 , 5 - bisphosphate
1. We have developed methods that yield estimates of the 32p content of each of the individual phosphate groups of phosphatidylinositol 4-phosphate and phosphatidylinositol 4,5-bisphosphate, thus extending the information available from studies of the labelling of these lipids in intact cells or membrane preparations. The analyses are undertaken with the deacylated lipids. 2. Assay of the 5-pho...
متن کاملRecognizing Phosphatidylinositol 3-Phosphate
Phosphatidylinositol 3-phosphate directs the endosomal localization of regulatory proteins by binding to FYVE and PX domains. New structures of these domains complexed with the phosphoinositide headgroup show how interactions with phosphate and hydroxyl groups differentiate this lipid from all others.
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ژورنال
عنوان ژورنال: Biological and Pharmaceutical Bulletin
سال: 2000
ISSN: 0918-6158,1347-5215
DOI: 10.1248/bpb.23.1088